Exclusive Services

CyclomicsSeq: Ultra-Accurate Sequencing of Short and Long Amplicons

CyclomicsSeq solves the challenge of finding the proverbial needle in a haystack in the genomic world. Designed for ultra-sensitive detection, CyclomicsSeq is the ideal technology for researchers and clinicians searching for rare genetic variants, especially those associated with cancer. Whether you are tracking minute traces of circulating tumor DNA or identifying low-frequency mutations in a complex sample, CyclomicsSeq delivers unparalleled accuracy and depth, making it the perfect solution for applications where precision is critical.

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Key Features:

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Unmatched Sensitivity and Accuracy: CyclomicsSeq delivers Q40 consensus accuracy, providing confidence in every result and reducing false positives and false negatives in biomarker detection.

Broad Compatibility: Capable of handling a wide range of DNA lengths (50bp to 3kb), CyclomicsSeq accommodates various amplicons and genetic targets, making it versatile for both clinical diagnostics and exploratory research.

High Throughput: With the ability to generate up to 50 million high-quality reads per run, CyclomicsSeq supports large-scale studies, offering both speed and precision for researchers and healthcare providers.

MeCy: Methylation Analysis Without Bisulfite Treatment

MeCy is Cyclomics' groundbreaking technology for CpG island methylation analysis, offering a simpler, more efficient approach by eliminating the need for traditional bisulfite conversion. This patent-pending technology preserves the integrity of the DNA while delivering high-accuracy methylation profiles without the harsh chemical treatments typically required. MeCy is compatible with both long-read and short-read sequencing platforms, making it versatile for various research and clinical applications. Additionally, MeCy goes beyond just methylation analysis—it provides single nucleotide polymorphism (SNP) and copy number variation (CNV) data in a single sequencing run, offering comprehensive insights into genomic and epigenomic alterations.

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Key Features:

Efficient and Accurate: By eliminating bisulfite conversion, MeCy simplifies the workflow, reduces DNA damage, and produces more reliable methylation data.

Platform Compatibility: MeCy works seamlessly with both long-read platforms like Nanopore sequencing and short-read platforms like Illumina, giving researchers flexibility based on their preferred technology.

Comprehensive Data in One Run: MeCy simultaneously detects SNPs, CNVs, and methylation patterns, offering an integrated solution for understanding complex genetic and epigenetic changes.

PlasmidSeq: High-Resolution Plasmid Sequencing

PlasmidSeq is Cyclomics' advanced solution for sequencing entire plasmids with single-molecule resolution. As plasmids are the natural substrate for Rolling Circle Amplification (RCA), we leverage our deep expertise in RCA and consensus generation to deliver high-quality, end-to-end plasmid sequencing. This technology provides unparalleled accuracy, ensuring comprehensive plasmid analysis from start to finish. Whether you need to verify plasmid constructs, analyze complex plasmid libraries, or sequence unknown vectors, PlasmidSeq delivers precise and reliable results tailored to your needs.

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Key Applications:

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Quality Control of Plasmid Libraries: PlasmidSeq is perfect for quality control (QC) analysis of large plasmid libraries, where high accuracy is essential to ensure that each plasmid construct is correctly assembled and free from errors. Researchers working on gene therapies, molecular cloning, or protein expression can rely on PlasmidSeq for precise verification of their plasmid constructs.

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Monoclonal Verification: In the development of monoclonal plasmid clones, precise sequencing is critical to ensure that the desired insert and vector backbone are correctly assembled without mutations. PlasmidSeq provides high-resolution sequencing for this purpose, delivering reliable data to confirm the accuracy of your monoclonal plasmid constructs.

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De-Novo Sequencing of Unknown Vectors: PlasmidSeq excels in de-novo sequencing, making it an ideal tool for identifying unknown or novel plasmid vectors. Whether you're working with uncharacterized plasmids from bacterial samples or creating custom vectors for synthetic biology, PlasmidSeq can provide a complete and detailed sequence of the plasmid, even if its structure is unknown.

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Mutation Analysis in Engineered Vectors: For researchers engineering plasmids for CRISPR, gene editing, or synthetic biology applications, ensuring that the plasmid sequences are accurate and free from unintended mutations is crucial. PlasmidSeq allows for the in-depth analysis of engineered vectors to identify point mutations, insertions, deletions, or rearrangements, giving you confidence in your constructs.

Nanopore Sequencing: Expertise and Excellence

At Cyclomics, we bring years of experience and deep expertise in molecular biology, Nanopore sequencing, and bioinformatics to deliver top-notch sequencing solutions tailored to your needs. Our team has a proven track record of successfully handling a wide range of sequencing projects, from straightforward "vanilla" sequencing runs to complex, large-scale genomic studies. Whether you're working on basic research, clinical diagnostics, or cutting-edge biotech applications, we have the knowledge and skills to ensure that your sequencing data is accurate, high-quality, and delivered on time.

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Why Choose Cyclomics for Nanopore Sequencing?

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Pluriennial Expertise: Our team’s extensive experience with Nanopore sequencing allows us to offer unmatched precision and adaptability, ensuring that even the most complex projects are executed with excellence.

Comprehensive Solutions: From simple sequencing tasks to advanced multi-omics and bioinformatics-driven projects, we handle it all. Whether you need a basic run or a custom pipeline for complex genomic analysis, Cyclomics is your partner.

Tailored Approaches: We work closely with our clients to understand their specific requirements, providing customized sequencing solutions that meet your goals, timeline, and budget.

Key Applications:

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Standard Sequencing Runs: For projects requiring reliable and high-throughput sequencing, we offer "vanilla" Nanopore runs that deliver fast, accurate results with minimal intervention.

Complex Genomic Studies: If you’re tackling more challenging projects—such as whole-genome sequencing, metagenomics, or transcriptomics—our team has the bioinformatics expertise and sequencing infrastructure to support every step of your workflow.

Human cfDNA Sequencing: Two Powerful Approaches

Cyclomics offers two distinct approaches for cfDNA sequencing and analysis, each tailored to provide high-resolution insights into circulating cell-free DNA (cfDNA). Both approaches enable multi-omics data extraction, delivering layers of information from genetic mutations to epigenetic modifications, ensuring comprehensive genomic and epigenomic analysis.

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NanoRCS
NanoRCS provides highly accurate consensus reads (Q40) with 1x-3x coverage across the human genome. This approach is ideal for detecting rare genetic mutations and structural variations. By producing high-quality reads, NanoRCS ensures reliable and trustworthy data for both research and clinical applications.

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Key Features:

Q40 Consensus Reads: Ultra-accurate, high-quality sequencing data for detailed genetic analysis.

1x-3x Genome Coverage: Low-coverage sequencing that balances accuracy with sufficient breadth for whole-genome analysis.​​

Naive cfDNA: High-Coverage, Methylation and Modification Insights

The Naive cfDNA approach delivers Q20-25 reads but achieves higher coverage at 10x, allowing for more in-depth exploration of cfDNA. This method is particularly suited for applications requiring detailed methylation analysis and base modification detection, offering both genetic and epigenetic insights.

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Key Features:

Q20-25 Reads: Moderate accuracy but high coverage, ensuring a deep dive into cfDNA.

10x Genome Coverage: High-coverage sequencing for comprehensive analysis.

Methylation and Base Modifications: Detects methylation patterns and other epigenetic modifications, making it valuable for cancer epigenomics and disease monitoring.